{
 "cells": [
  {
   "cell_type": "code",
   "execution_count": 52,
   "id": "9a6cbb9c-2521-4f01-8c83-d653ade653df",
   "metadata": {},
   "outputs": [],
   "source": [
    "import numpy as np\n",
    "import pandas as pd\n",
    "from pandas import DataFrame, Series  # for convenience\n",
    "from skimage import morphology, util, filters\n",
    "from skimage.transform import warp_polar\n",
    "\n",
    "import pims\n",
    "import trackpy as tp\n",
    "\n",
    "import matplotlib as mpl\n",
    "import matplotlib.pyplot as plt\n",
    "\n",
    "# Optionally, tweak styles.\n",
    "mpl.rc('figure',  figsize=(10, 5))\n",
    "mpl.rc('image', cmap='gray')"
   ]
  },
  {
   "cell_type": "markdown",
   "id": "3dac9f80-5932-43a3-a588-6eea8c202d61",
   "metadata": {},
   "source": [
    "## Function for calculating particle centroid from a brightfield ring. "
   ]
  },
  {
   "cell_type": "markdown",
   "id": "a05c8afc-385b-4ff9-a232-82512f43f02a",
   "metadata": {},
   "source": [
    "Only need to load this once after starting or restarting the kernel. You can also make a separate python functions file and import that above"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 53,
   "id": "9efa81b1-2150-460c-aaa7-759192258c29",
   "metadata": {},
   "outputs": [],
   "source": [
    "from scipy.optimize import least_squares\n",
    "from skimage.transform import warp_polar\n",
    "def calc_centroid_brightfield(img,c0,r0,rbound,mask_r):\n",
    "    \"\"\"\n",
    "    Calculates centroid of a particle by fitting a circle to the darkest ring around the particle. Note in the raw image, the brightest ring is usually what \n",
    "    is fit, so the image is passed inverted. \n",
    "    From the initial guess of the centroid c0, the pixels < rbound around c0 are transformed to polar coordinates using skimage.transform.warp_polar\n",
    "    Then, for each degree (warp_polar produces 360 rows/degrees), the boundary of the ring is estimated as the darkest point along that radial coordinate\n",
    "    See SI Figure S2 of DOI:10.1039/C8SM01661E for the high-level picture of how this algorithm works\n",
    "    REQUIRES: img - frame/image\n",
    "              c0 - list of tuples of initial guess of particle centroids [(cx00,cy00),(cx01,cy01),...,(cx0N,cy0N)] for N particles\n",
    "              r0 - initial guess of the radius of the darkest ring around the particle, units of pixels\n",
    "              rbound - maximum radius around c0 to search for the dark ring, units of pixels\n",
    "    RETURNS: list of particle centroid coordinates and radius, [cx,cy,r]\n",
    "    \"\"\"\n",
    "\n",
    "    def fit_fxn(vars,xdata,ydata):\n",
    "        cx = vars[0]\n",
    "        cy = vars[1]\n",
    "        r = vars[2]\n",
    "        #calculate residuals of radial positions from true radius\n",
    "        radii = np.sqrt((xdata - cx)**2 + (ydata - cy)**2) - r\n",
    "        return radii\n",
    "    # find the particle boundary radially by converting to polar coordinates. Manually select initial guess interactively, then use guesses from previous frames\n",
    "    # choose radius that is larger than the particle size. The radius bounds the region where the particle intensity is compared\n",
    "    cx0 = c0[j][0]\n",
    "    cy0 = c0[j][1]\n",
    "\n",
    "    warped = warp_polar(img,center=(cy0,cx0),radius = rbound) #warping ix (y,x)\n",
    "    theta = np.linspace(0,359,360)\n",
    "\n",
    "    #CONSTRUCT PARTICLE RING. \n",
    "    #this part of the code is something you could change in the future... e.g. could have multiple characteristic points per angle \n",
    "    #estimate dark ring by the minimum brightness (darkest point for any given radial position)\n",
    "    #mask points near the centroid by making them bright...in some images the center of the particle is brightest which throws off calculation\n",
    "    if mask_r != 0 :\n",
    "        warped[:,0:mask_r] = 255\n",
    "    dark_ring = np.argmin(warped,axis=1)\n",
    "    #estimate bright ring by the maximum brightness (lightest/whitest point for any given radial position)\n",
    "    #bright_ring = np.argmax(warped,axis=1)\n",
    "    \n",
    "    #Convert these points back into x,y data    \n",
    "    xdata = dark_ring*np.cos(theta*np.pi/180)+cx0\n",
    "    ydata = dark_ring*np.sin(theta*np.pi/180)+cy0\n",
    "\n",
    "    #now that you have an estimate of the particle outline in cartesian, use least squares to determine the origin and radius. We care mostly about the origin\n",
    "    lsq_result = least_squares(fit_fxn,[cx0,cy0,r0],args=([xdata,ydata]),loss='soft_l1')\n",
    "    cx = lsq_result.x[0]\n",
    "    cy = lsq_result.x[1]\n",
    "    r = lsq_result.x[2]\n",
    "\n",
    "    return [cx,cy,r]"
   ]
  },
  {
   "cell_type": "markdown",
   "id": "51117400-3a8a-459d-b359-c8e98845ad50",
   "metadata": {},
   "source": [
    "## Load in video folder and setup some basic preprocessing "
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 54,
   "id": "36a9f5f6-f67f-484e-ae57-d05a7dc6ff98",
   "metadata": {},
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "Found 4 speed folders: ['0.1', '0.15', '0.2', '0.25']\n",
      "  [0.1]  154 frames,  shape: (1464, 1936)\n",
      "  [0.15]  135 frames,  shape: (1464, 1936)\n",
      "  [0.2]  133 frames,  shape: (1464, 1936)\n",
      "  [0.25]  140 frames,  shape: (1464, 1936)\n",
      "\n",
      "Done. Access a speed with:  all_frames['0.25']\n"
     ]
    },
    {
     "name": "stderr",
     "output_type": "stream",
     "text": [
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n"
     ]
    }
   ],
   "source": [
    "import os\n",
    "\n",
    "# =============================================================================\n",
    "# SETTINGS\n",
    "# =============================================================================\n",
    "MAIN_FOLDER    = r\"/Users/mpitell/Downloads/Drag tracking test\"\n",
    "TIFF_PATTERN   = \"*.tiff\"          # glob pattern for images inside each subfolder\n",
    "crop_dimensions = {'x_min': 800, 'x_max': 1100, 'y_min': 300, 'y_max': 1200}\n",
    "# =============================================================================\n",
    "\n",
    "@pims.pipeline\n",
    "def to_gray(image):\n",
    "    if image.ndim == 3:\n",
    "        return image[..., 1]\n",
    "    return image\n",
    "\n",
    "@pims.pipeline\n",
    "def crop(img, crop_dimensions):\n",
    "    return img[crop_dimensions['y_min']:crop_dimensions['y_max'],\n",
    "               crop_dimensions['x_min']:crop_dimensions['x_max']]\n",
    "\n",
    "@pims.pipeline\n",
    "def invert(img):\n",
    "    return 255 - img\n",
    "\n",
    "# --- Discover subfolders (each = one stage speed) ---\n",
    "subfolders = sorted([\n",
    "    d for d in os.listdir(MAIN_FOLDER)\n",
    "    if os.path.isdir(os.path.join(MAIN_FOLDER, d))\n",
    "])\n",
    "print(f\"Found {len(subfolders)} speed folders: {subfolders}\")\n",
    "\n",
    "# --- Load and preprocess all folders ---\n",
    "# all_frames[speed] = preprocessed (gray + crop) pims pipeline\n",
    "# all_frames_inv[speed] = inverted version (used during tracking)\n",
    "all_frames     = {}\n",
    "all_frames_inv = {}\n",
    "\n",
    "for speed in subfolders:\n",
    "    path   = os.path.join(MAIN_FOLDER, speed, TIFF_PATTERN)\n",
    "    raw    = pims.open(path)\n",
    "    gray   = to_gray(raw)\n",
    "    cropped = crop(gray, crop_dimensions)\n",
    "    all_frames[speed]     = cropped\n",
    "    all_frames_inv[speed] = invert(cropped)\n",
    "    print(f\"  [{speed}]  {len(raw)} frames,  shape: {raw.frame_shape}\")\n",
    "\n",
    "print(\"\\nDone. Access a speed with:  all_frames['0.25']\")"
   ]
  },
  {
   "cell_type": "markdown",
   "id": "d705e72d-863d-471f-92df-987bb634eef9",
   "metadata": {},
   "source": [
    "## Setup for particle tracking"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 55,
   "id": "6c850cd3-d1d4-4734-9454-58906cb14878",
   "metadata": {},
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "Frame ranges per speed:\n",
      "  [0.1]  frames 0 – 153  (154 total)\n",
      "  [0.15]  frames 0 – 134  (135 total)\n",
      "  [0.2]  frames 0 – 132  (133 total)\n",
      "  [0.25]  frames 0 – 139  (140 total)\n"
     ]
    }
   ],
   "source": [
    "num_particles    = 3   # number of particles to track\n",
    "particle_r_bound = 50  # bounding radius around particle when determining centroid\n",
    "mask_r           = 10  # masks bright region near particle center\n",
    "r0               = 35  # initial guess of bright ring radius\n",
    "\n",
    "# Start/end frames auto-detected per speed from the loaded image sequences\n",
    "frame_ranges = {\n",
    "    speed: (0, len(all_frames[speed]) - 1)\n",
    "    for speed in all_frames\n",
    "}\n",
    "\n",
    "# ploc_dict[speed] = 3D array (frames × particles × [cx, cy, r])\n",
    "ploc_dict = {\n",
    "    speed: np.zeros((end - start + 1, num_particles, 3))\n",
    "    for speed, (start, end) in frame_ranges.items()\n",
    "}\n",
    "\n",
    "print(\"Frame ranges per speed:\")\n",
    "for speed, (start, end) in frame_ranges.items():\n",
    "    print(f\"  [{speed}]  frames {start} – {end}  ({end - start + 1} total)\")"
   ]
  },
  {
   "cell_type": "markdown",
   "id": "e8b3789e-84be-44b8-8e0d-02cd49012138",
   "metadata": {},
   "source": [
    "### manually hover over interactive plot to specify initial guesses of the particle centroids"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 62,
   "id": "abbd2f16-1502-490d-b075-b3246381017e",
   "metadata": {},
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "Speeds: ['0.1', '0.15', '0.2', '0.25']\n",
      "Click 3 center(s) per image. Each speed opens its own figure.\n",
      "\n"
     ]
    },
    {
     "name": "stderr",
     "output_type": "stream",
     "text": [
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n"
     ]
    },
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      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n"
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      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n"
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      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n"
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   ],
   "source": [
    "%matplotlib widget\n",
    "\n",
    "import matplotlib.pyplot as plt\n",
    "\n",
    "speeds  = list(all_frames.keys())\n",
    "c0_dict = {s: [] for s in speeds}\n",
    "figs    = {}\n",
    "\n",
    "print(f\"Speeds: {speeds}\")\n",
    "print(f\"Click {num_particles} center(s) per image. Each speed opens its own figure.\\n\")\n",
    "\n",
    "def make_click_handler(speed, fig):\n",
    "    def on_click(event):\n",
    "        if event.inaxes is None or event.button != 1:\n",
    "            return\n",
    "        pts = c0_dict[speed]\n",
    "        if len(pts) >= num_particles:\n",
    "            print(f\"[{speed}] already full — ignoring\")\n",
    "            return\n",
    "        x, y = event.xdata, event.ydata\n",
    "        pts.append((x, y))\n",
    "        event.inaxes.plot(x, y, \"r+\", markersize=14, markeredgewidth=2)\n",
    "        event.inaxes.set_title(f\"speed = {speed}  ({len(pts)}/{num_particles})\", fontsize=11)\n",
    "        fig.canvas.draw_idle()\n",
    "        print(f\"[{speed}] click {len(pts)}/{num_particles}: ({x:.1f}, {y:.1f})\")\n",
    "        if all(len(v) == num_particles for v in c0_dict.values()):\n",
    "            print(\"\\nAll speeds complete! Run the confirm cell.\")\n",
    "    return on_click\n",
    "\n",
    "for speed in speeds:\n",
    "    fig, ax = plt.subplots(figsize=(6, 5))\n",
    "    ax.imshow(all_frames[speed][0], cmap=\"gray\")\n",
    "    ax.set_title(f\"speed = {speed}  (0/{num_particles})\", fontsize=11)\n",
    "    ax.axis(\"off\")\n",
    "    fig.canvas.mpl_connect(\"button_press_event\", make_click_handler(speed, fig))\n",
    "    fig.tight_layout()\n",
    "    figs[speed] = fig\n",
    "    plt.show()"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 63,
   "id": "b5df80a4-318b-478a-ad46-730b938adc5a",
   "metadata": {},
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "[0.1]  3 point(s): [(141.5, 287.0), (111.5, 503.2), (93.5, 737.4)]\n",
      "[0.15]  3 point(s): [(141.5, 285.0), (119.5, 491.2), (85.5, 733.4)]\n",
      "[0.2]  3 point(s): [(139.5, 277.0), (117.5, 493.2), (89.5, 733.4)]\n",
      "[0.25]  3 point(s): [(145.5, 283.0), (109.5, 501.2), (85.5, 743.4)]\n"
     ]
    }
   ],
   "source": [
    "# Confirm collected c0 values — run after clicking all panels above.\n",
    "# c0_dict[speed] is a list of (x, y) tuples in the order you clicked.\n",
    "\n",
    "for speed, pts in c0_dict.items():\n",
    "    print(f\"[{speed}]  {len(pts)} point(s): {[(round(x,1), round(y,1)) for x,y in pts]}\")\n",
    "\n",
    "if any(len(v) != num_particles for v in c0_dict.values()):\n",
    "    print(\"\\nWARNING: some speeds have the wrong number of points. \"\n",
    "          \"Re-run the previous cell to redo clicks.\")"
   ]
  },
  {
   "cell_type": "markdown",
   "id": "ce6c3145-62c4-48cd-bf48-3f71ff67daba",
   "metadata": {},
   "source": [
    "# Locate particles "
   ]
  },
  {
   "cell_type": "markdown",
   "id": "cf67e214-319a-4bf8-b9c4-0705624bbe7f",
   "metadata": {},
   "source": [
    "Produces 3D numpy array of particle locations with row index being the frame number, column index the particle number, and z index xcoord [0], ycoord [1], radius [2] of ring fit to particle. "
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 64,
   "id": "74824cdc-b3c0-4193-aaa6-dae3793bfa03",
   "metadata": {},
   "outputs": [
    {
     "name": "stderr",
     "output_type": "stream",
     "text": [
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n"
     ]
    },
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "[0.1]  test run done (frames 0–9)\n"
     ]
    },
    {
     "name": "stderr",
     "output_type": "stream",
     "text": [
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n"
     ]
    },
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "[0.15]  test run done (frames 0–9)\n"
     ]
    },
    {
     "name": "stderr",
     "output_type": "stream",
     "text": [
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n"
     ]
    },
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "[0.2]  test run done (frames 0–9)\n",
      "[0.25]  test run done (frames 0–9)\n",
      "\n",
      "Done. Run the verify cell to check results.\n"
     ]
    },
    {
     "name": "stderr",
     "output_type": "stream",
     "text": [
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n"
     ]
    }
   ],
   "source": [
    "# --- TEST RUN: first 10 frames only ---\n",
    "# Check the verify cell below before running the full tracking.\n",
    "\n",
    "TEST_FRAMES = 10\n",
    "\n",
    "for speed in speeds:\n",
    "    start, end = frame_ranges[speed]\n",
    "    test_end   = min(start + TEST_FRAMES - 1, end)\n",
    "\n",
    "    c0 = list(c0_dict[speed])   # copy so we don't mutate c0_dict\n",
    "    r0_run = r0\n",
    "\n",
    "    for i in range(start, test_end + 1):\n",
    "        for j in range(num_particles):\n",
    "            ploc_dict[speed][i, j, :] = calc_centroid_brightfield(\n",
    "                all_frames_inv[speed][i], c0, r0_run, particle_r_bound, mask_r)\n",
    "            c0[j]  = (ploc_dict[speed][i, j, 0], ploc_dict[speed][i, j, 1])\n",
    "            r0_run =  ploc_dict[speed][i, j, 2]\n",
    "\n",
    "    print(f\"[{speed}]  test run done (frames {start}–{test_end})\")\n",
    "\n",
    "print(\"\\nDone. Run the verify cell to check results.\")"
   ]
  },
  {
   "cell_type": "markdown",
   "id": "f878e6d3-a592-4eea-8b58-d418237252f0",
   "metadata": {},
   "source": [
    "## Verify result for a given frame"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 65,
   "id": "33769150-1338-45df-861c-4fda50583f00",
   "metadata": {},
   "outputs": [
    {
     "name": "stderr",
     "output_type": "stream",
     "text": [
      "/var/folders/89/5qr75h815xjgjwn9r2_klvpw0000gp/T/ipykernel_32448/4217944463.py:10: RuntimeWarning: More than 20 figures have been opened. Figures created through the pyplot interface (`matplotlib.pyplot.figure`) are retained until explicitly closed and may consume too much memory. (To control this warning, see the rcParam `figure.max_open_warning`). Consider using `matplotlib.pyplot.close()`.\n",
      "  fig, ax = plt.subplots(figsize=(6, 5))\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n"
     ]
    },
    {
     "data": {
      "application/vnd.jupyter.widget-view+json": {
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      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n"
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      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n"
     ]
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       "\n",
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       "                    Figure\n",
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    {
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     "output_type": "stream",
     "text": [
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n"
     ]
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       "\n",
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       "                <div class=\"jupyter-widgets widget-label\" style=\"text-align: center;\">\n",
       "                    Figure\n",
       "                </div>\n",
       "                <img src='data:image/png;base64,[encoded data, 91772 characters]' width=600.0/>\n",
       "            </div>\n",
       "        "
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   ],
   "source": [
    "# Verify frame 0 for every speed — fitted ring overlaid on the image.\n",
    "# If the circles look wrong, adjust c0_dict clicks or tracking parameters and re-run the test.\n",
    "\n",
    "%matplotlib widget\n",
    "\n",
    "frame_num = 0\n",
    "theta = np.linspace(0, 359, 360)\n",
    "\n",
    "for speed in speeds:\n",
    "    fig, ax = plt.subplots(figsize=(6, 5))\n",
    "    ax.imshow(all_frames_inv[speed][frame_num], cmap=\"gray\")\n",
    "    ax.set_title(f\"speed = {speed}  |  frame {frame_num}\", fontsize=11)\n",
    "    ax.axis(\"off\")\n",
    "    for i in range(num_particles):\n",
    "        cx = ploc_dict[speed][frame_num, i, 0]\n",
    "        cy = ploc_dict[speed][frame_num, i, 1]\n",
    "        r  = ploc_dict[speed][frame_num, i, 2]\n",
    "        ax.plot(r * np.cos(theta * np.pi / 180) + cx,\n",
    "                r * np.sin(theta * np.pi / 180) + cy, lw=1.5)\n",
    "        ax.plot(cx, cy, \"+\", markersize=8, markeredgewidth=1.5)\n",
    "    fig.tight_layout()\n",
    "    plt.show()"
   ]
  },
  {
   "cell_type": "markdown",
   "id": "9l6r1ih5xf5",
   "metadata": {},
   "source": [
    "## Full tracking — run after verifying test results above"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 60,
   "id": "jjlx2ak3lyr",
   "metadata": {},
   "outputs": [
    {
     "name": "stderr",
     "output_type": "stream",
     "text": [
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n"
     ]
    },
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "[0.1]  complete  (154 frames)\n"
     ]
    },
    {
     "name": "stderr",
     "output_type": "stream",
     "text": [
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n"
     ]
    },
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "[0.15]  complete  (135 frames)\n"
     ]
    },
    {
     "name": "stderr",
     "output_type": "stream",
     "text": [
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n"
     ]
    },
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "[0.2]  complete  (133 frames)\n"
     ]
    },
    {
     "name": "stderr",
     "output_type": "stream",
     "text": [
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n"
     ]
    },
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "[0.25]  complete  (140 frames)\n",
      "\n",
      "All speeds tracked. Run the output cell to save results.\n"
     ]
    },
    {
     "name": "stderr",
     "output_type": "stream",
     "text": [
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n",
      "/opt/anaconda3/lib/python3.12/site-packages/pims/image_sequence.py:85: FutureWarning: The plugin infrastructure in `skimage.io` and the parameter `plugin` are deprecated since version 0.25 and will be removed in 0.27 (or later). To avoid this warning, please do not use the parameter `plugin`. Instead, use `imageio` or other I/O packages directly. See also `imread`.\n",
      "  return imread(filename, **kwargs)\n"
     ]
    }
   ],
   "source": [
    "# --- FULL RUN over all frames for every speed ---\n",
    "# Only run this once the verify cell looks good.\n",
    "\n",
    "for speed in speeds:\n",
    "    start, end = frame_ranges[speed]\n",
    "    c0     = list(c0_dict[speed])\n",
    "    r0_run = r0\n",
    "\n",
    "    for i in range(start, end + 1):\n",
    "        for j in range(num_particles):\n",
    "            ploc_dict[speed][i, j, :] = calc_centroid_brightfield(\n",
    "                all_frames_inv[speed][i], c0, r0_run, particle_r_bound, mask_r)\n",
    "            c0[j]  = (ploc_dict[speed][i, j, 0], ploc_dict[speed][i, j, 1])\n",
    "            r0_run =  ploc_dict[speed][i, j, 2]\n",
    "\n",
    "    print(f\"[{speed}]  complete  ({end - start + 1} frames)\")\n",
    "\n",
    "print(\"\\nAll speeds tracked. Run the output cell to save results.\")"
   ]
  },
  {
   "cell_type": "markdown",
   "id": "1472939b-e353-4e17-9e78-0a8c98c8994c",
   "metadata": {},
   "source": [
    "## Notes"
   ]
  },
  {
   "cell_type": "markdown",
   "id": "b475b67d-3557-429d-a4a1-7579a5edaa30",
   "metadata": {},
   "source": [
    "For Matt: Think about what you want to consider the end product of the processing, and then save that output as a CSV/excel file. Then, make a new jupyter notebook for analysis that loads in that file. I found it useful when I processed my particle tracking to divide up the tasks for a few reasons\n",
    "1) It makes sure you're saving the processing somewhere (trust me, this is important).\n",
    "2) Provides a record of exactly the processing that was used in a given analysis\n",
    "3) Shortens the notebooks/makes them cleaner. "
   ]
  },
  {
   "cell_type": "markdown",
   "id": "2527b45b-f2a9-45d9-a460-f2471b7bf3e6",
   "metadata": {},
   "source": [
    "## Output particle centroids and radii numpy array, ploc, and a metadata file"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "id": "e97b383e-1f2d-43ed-a3d4-d68db0eaae4c",
   "metadata": {},
   "outputs": [
    {
     "data": {
      "application/vnd.jupyter.widget-view+json": {
       "model_id": "f9a3a95f9a054f9d80c42a80d4cd4e18",
       "version_major": 2,
       "version_minor": 0
      },
      "text/plain": [
       "VBox(children=(Text(value='/Users/mpitell/Downloads/Drag Tracking Output', description='Output folder:', layou…"
      ]
     },
     "metadata": {},
     "output_type": "display_data"
    }
   ],
   "source": [
    "import os\n",
    "import json\n",
    "import ipywidgets as widgets\n",
    "from IPython.display import display\n",
    "\n",
    "# --- Select output folder ---\n",
    "folder_box = widgets.Text(\n",
    "    value=os.path.expanduser(\"~/Downloads/Drag Tracking Output\"),\n",
    "    description=\"Output folder:\",\n",
    "    layout=widgets.Layout(width=\"500px\"),\n",
    "    style={\"description_width\": \"120px\"}\n",
    ")\n",
    "save_btn = widgets.Button(description=\"Save CSVs\", button_style=\"success\")\n",
    "status   = widgets.Output()\n",
    "\n",
    "display(widgets.VBox([folder_box, save_btn, status]))\n",
    "\n",
    "def on_save(_):\n",
    "    with status:\n",
    "        status.clear_output()\n",
    "        output_folder = folder_box.value.strip()\n",
    "        os.makedirs(output_folder, exist_ok=True)\n",
    "\n",
    "        for speed in speeds:\n",
    "            ploc = ploc_dict[speed]\n",
    "            start, end = frame_ranges[speed]\n",
    "\n",
    "            # Match reference format: no header, 9 cols (cx0,cy0,r0,cx1,cy1,r1,...)\n",
    "            # Filename: \"{speed} mms.csv\"\n",
    "            csv_path  = os.path.join(output_folder, f\"{speed} mms.csv\")\n",
    "            np.savetxt(csv_path, ploc.reshape(ploc.shape[0], -1), delimiter=\",\")\n",
    "\n",
    "            # Metadata JSON\n",
    "            meta = {\n",
    "                \"speed\":            speed,\n",
    "                \"num_particles\":    num_particles,\n",
    "                \"particle_r_bound\": particle_r_bound,\n",
    "                \"mask_r\":           mask_r,\n",
    "                \"r0\":               r0,\n",
    "                \"start_frame\":      start,\n",
    "                \"end_frame\":        end,\n",
    "                \"c0\":               c0_dict[speed]\n",
    "            }\n",
    "            json_path = os.path.join(output_folder, f\"{speed} mms.json\")\n",
    "            with open(json_path, \"w\") as f:\n",
    "                json.dump(meta, f, indent=4)\n",
    "\n",
    "            print(f\"[{speed}]  saved  →  {csv_path}\")\n",
    "\n",
    "        print(f\"\\nAll files saved to:  {output_folder}\")\n",
    "\n",
    "save_btn.on_click(on_save)"
   ]
  },
  {
   "cell_type": "markdown",
   "id": "d31fbaff-07c8-4b76-b353-4b18a459afe0",
   "metadata": {},
   "source": [
    "# Misc. and testing snippets"
   ]
  },
  {
   "cell_type": "markdown",
   "id": "e477e63e-4dd9-4f28-8180-95c707155677",
   "metadata": {},
   "source": [
    "## calculate displacement"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 11,
   "id": "d45a87fd-4a5d-4021-9872-945a78f4a595",
   "metadata": {},
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "[[0.05213566 0.09631768 0.06504412]\n",
      " [0.41345626 0.12543199 0.49766494]\n",
      " [0.58382393 0.31758089 0.00763203]\n",
      " [0.27727725 0.59071142 0.2290869 ]\n",
      " [0.38528757 0.09031707 0.4065928 ]\n",
      " [0.15265725 0.3862622  0.09598088]\n",
      " [0.56254089 0.1960431  0.35763945]\n",
      " [0.23060543 0.32493377 0.23188197]\n",
      " [0.15187648 0.40148314 0.12412066]\n",
      " [0.37393807 0.14021299 0.08339964]]\n"
     ]
    }
   ],
   "source": [
    "delta_d = np.zeros((np.size(ploc,0)-1,3))\n",
    "for i in range(np.size(ploc,0)-1):\n",
    "    delta_d[i,:] = np.sqrt(np.square(ploc[i+1,:,0] - ploc[i,:,0]) + np.square(ploc[i+1,:,1] - ploc[i,:,1]))\n",
    "print(delta_d)"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 28,
   "id": "51f54c2a",
   "metadata": {},
   "outputs": [],
   "source": [
    "import os\n",
    "import json\n",
    "import numpy as np\n",
    "\n",
    "output_folder = r\"C:\\Users\\matth\\Documents\\Drag Tracking\"\n",
    "outFileName = 'tracking' #without file extension\n",
    "np.savetxt(\n",
    "    os.path.join(output_folder, outFileName + \".csv\"),\n",
    "    ploc.reshape(ploc.shape[0], -1),   # collapse extra dimensions\n",
    "    delimiter=\",\"\n",
    ")\n",
    "output_dict = {'num_particles':num_particles,'particle_r_bound':particle_r_bound,'mask_r': mask_r,'r0': r0,'start_frame':start_frame,'end_frame':end_frame}\n",
    "with open(os.path.join(output_folder,(outFileName +'.json')),'w') as fout:\n",
    "    json.dump(output_dict,fout,indent=4)"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "id": "e211efd7",
   "metadata": {},
   "outputs": [],
   "source": []
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "id": "1f0a8209",
   "metadata": {},
   "outputs": [],
   "source": []
  }
 ],
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